Science signaling podcast: 2 february 2010.
Authors: Kino T, Vanhook AM
This is a conversation with Tomoshige Kino about a Research Article published in the 2 February 2010 issue of Science Signaling.
PMID: 20124550 [PubMed - in process] (Source: Science Signaling)...
POSTED 02/05/2010 at 07:14 PM --
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Noncoding RNA gas5 is a growth arrest- and starvation-associated repressor of the glucocorticoid receptor.
Authors: Kino T, Hurt DE, Ichijo T, Nader N, Chrousos GP
The availability of nutrients influences cellular growth and survival by affecting gene transcription. Glucocorticoids also influence gene transcription and have diverse activities on cell growth, energy expenditure, and survival. We found that the growth arrest-specific 5 (Gas5) noncoding RNA, which is abundant in cells whose growth has been arrested because of lack of nutrients or growth factors, sensitized cells to apoptosis by suppressing glucocorticoid-mediated induction of several responsive genes, including the one encoding cellular inhibitor of apoptosis 2. Gas5 bound to the DNA-binding domain of the glucocorticoid receptor (GR) by acting as a decoy glucocorticoid response element (GRE), thus competing with DNA GREs for b......
POSTED 02/05/2010 at 07:14 PM --
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By tracking water molecules, physicists hope to unlock secrets of life
Compared to any other liquid on Earth, water behaves in strange and unexpected ways, yet its unusual properties enable and protect life as we know it. By tracking individual water molecules in a supercooled state, scientists find what explains one of waters most notable and life-saving features: its astounding capacity to resist gaining or losing heat. (Source: The Rockefeller University Newswire)...
POSTED 02/05/2010 at 04:00 PM --
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Newly engineered enzyme is a powerful staph antibiotic
In the past decade, methicillin-resistant Staphylococcus aureus, or MRSA, has ushered in a new era in the fight between man and bug. By harnessing the power of natures own antibiotics, scientists have engineered an enzyme known as a lysin that not only kills MRSA in mice but also works synergistically with antibiotics that were once powerless against the formidable organism. (Source: The Rockefeller University Newswire)...
POSTED 02/05/2010 at 01:30 PM --
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Sensitivity analysis and parametric study of elastic properties of an unidirectional mineralized bone fibril-array using mean field methods.
This study provides an improved insight in the parameters, which govern the fibril-array stiffness of mineralized tissues such as bone.
PMID: 20135190 [PubMed - as supplied by publisher] (Source: Biomechanics and Modeling in Mechanobiology)...
POSTED 02/04/2010 at 06:00 PM --
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Study of nobiletin binding to bovine serum albumin by capillary electrophoresis-frontal analysis and circular dichroism.
Authors: Yi L, Li H, Deng Q, Yuan Z
A very recent epidemiological study provided strong support for nobiletin (NOB) as a potential candidate chemopreventive agent against cancer. From the pharmacology point of view, drug-protein interactions are determining factors in therapeutic, pharmacodynamic and toxicological drug properties. In this work, for the first time, detection of NOB at near-physiological conditions was accomplished by means of capillary electrophoresis-frontal analysis (CE-FA), and then the binding constants of NOB with bovine serum albumin (BSA) at the same conditions were determined. Complexation of NOB-BSA led to a decrease of the height for free NOB with increasing concentration of BSA. These results revealed the presence of a single class of binding site on BSA, and......
POSTED 02/04/2010 at 06:00 PM --
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Increased plasma concentrations of anterior gradient 2 protein are positively associated with ovarian cancer
Ovarian cancer is often asymptomatic and is diagnosed at an advanced stage with poor survival rates, thus, there is an urgent need to develop biomarkers for earlier detection of ovarian cancer. Here, we demonstrate for the first time that the previously-reported metastasis-inducing protein, anterior gradient protein 2 (AGR2), can be detected in the blood of ovarian cancer patients. Using a newly developed ELISA test, we show significantly increased concentrations of AGR2 protein in plasma from cancer patients relative to normal controls. Plasma AGR2 concentrations were highest in stage II and stage III ovarian cancer patients and were similarly elevated in patients with both serous and non-serous tumours. The identification of elevated plasma concentrations of AGR2 may provide a useful bio......
POSTED 02/04/2010 at 06:00 PM --
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Cars of the future could be powered by their bodywork thanks to new battery technology
Scientists develop new composite material that could revolutionise car design and manufacturing – News Release (Source: Imperial College News)...
POSTED 02/04/2010 at 06:00 PM --
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Administration of a low dose of sildenafil for one week decreases intrahepatic resistance in rats with biliary cirrhosis: The role of NO bioavailability
Conclusions: Sildenafil treatment improves endothelial dysfunction by augmenting NO bioavailability in hepatic microcirculation. (Source: Clinical Science)...
POSTED 02/04/2010 at 06:00 PM --
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Discovery of Compounds That Bind to HIV Protease Could Lay Foundation for New Drugs
(Source: NIGMS - Results)...
POSTED 02/04/2010 at 01:51 PM --
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Chemists Clarify Exactly How Antiviral Drugs Block Influenza Viruses From Spreading
(Source: NIGMS - Results)...
POSTED 02/04/2010 at 01:49 PM --
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Mechanical Forces as Well as Biochemistry Appear to Affect Gene Expression
(Source: NIGMS - Results)...
POSTED 02/04/2010 at 01:47 PM --
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Some morbidly obese people are missing genes, shows new research
A segment of 30 missing genes may be responsible for the weight gain of some morbidly
obese people - News Release (Source: Imperial College News)...
POSTED 02/03/2010 at 06:00 PM --
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Isolation and cultivation of human keratinocytes from skin or plucked hair for the generation of induced pluripotent stem cells
Authors: Trond Aasen & Juan Carlos Izpisúa Belmonte
The ease of generating induced pluripotent stem (iPS) cells, and possibly their properties after reprogramming, depends on the origin of the somatic cell starting population. Reprogramming of keratinocytes is both faster and more efficient compared with fibroblasts, although more care is required when isolating, culturing (Source: Nature Protocols)...
POSTED 02/03/2010 at 06:00 PM --
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Multiplexed immunophenotyping of human antigen-presenting cells in whole blood by polychromatic flow cytometry
We describe two modular protocols for immunostaining and multiparameter flow cytometric analysis of major human antigen-presenting cells (APCs; e.g., dendritic cells, monocytes and B lymphocytes) in minimally manipulated whole blood samples. Simultaneous detection of up to eight colors is enabled by careful selection and testing (Source: Nature Protocols)...
POSTED 02/03/2010 at 06:00 PM --
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Construction and testing of engineered zinc-finger proteins for sequence-specific modification of mtDNA
Authors: Michal Minczuk, Paulina Kolasinska-Zwierz, Michael P Murphy & Monika A Papworth
Engineered zinc-finger proteins (ZFPs) are hybrid proteins developed to direct various effector domains (EDs) of choice to predetermined DNA sequences. They are used to alter gene expression and to modify DNA in a sequence-specific manner in vivo and in vitro. Until now, ZFPs (Source: Nature Protocols)...
POSTED 02/03/2010 at 06:00 PM --
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Using Trawler_standalone to discover overrepresented motifs in DNA and RNA sequences derived from various experiments including chromatin immunoprecipitation
Authors: Yannick Haudry, Mirana Ramialison, Benedict Paten, Joachim Wittbrodt & Laurence Ettwiller
Genome-wide location analysis has become a standard technology to unravel gene regulation networks. The accurate characterization of nucleotide signatures in sequences is key to uncovering the regulatory logic but remains a computational challenge. This protocol describes how to best characterize these signatures (motifs) using the (Source: Nature Protocols)...
POSTED 02/03/2010 at 06:00 PM --
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How Science Works: Evolution (Ellis)
A Student Primer Evolution is just a theory, isn’t it? What is a scientific theory anyway? Don’t scientists prove things? What is the difference between a fact, a hypothesis and a theory in science? How does scientific thinking differ from religious thinking? Why are most leading scientists atheists? Are science and religion compatible? Why are there so many different religious beliefs but only one ... (Source: Springer Biomedical Sciences titles)...
POSTED 02/03/2010 at 01:38 AM --
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NMR structure note: UBA domain of CIP75.
Authors: Kieken F, Spagnol G, Su V, Lau AF, Sorgen PL
PMID: 20127391 [PubMed - as supplied by publisher] (Source: Journal of Bimolecular NMR)...
POSTED 02/02/2010 at 06:00 PM --
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Using Surfactants With Anesthetic Stops Pain Without Affecting Muscle Function
(Source: NIGMS - Results)...
POSTED 02/02/2010 at 03:13 PM --
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